Clinical Significance of Coagulation Indicators and their Correlation with Inflammatory Factors in Critical Patients with Thromboembolism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To evaluate the levels of coagulation indicators [thrombomodulin(TM)/ thrombin-antithrombin complexes(TAT)/ α-plasmin inhibitor-plasmin complexes(PIC)/ tissue plasminogen activator-inhibitor complexes(t-PAIC) /D-Dimer(D-D)/fibrin degradation products(FDP)] in the critical patients with thromboembolism, analyse their correlation with inflammatory factor (procalcitonin/C reactive protein/ interleukin-6), and explore the diagnostic significance of coagulation indicators for these patients.</p><p><b>METHODS</b>The serum levels of the coagulation indicators (TM/TAT/PIC/t-PAIC/D-D /FDP) and inflammatory factors (PCT/IL-6/CRP) were detected in the patient group with critical thromboembolism (n= 38) and critical patient group without thromboembolism as control (n= 81) . The correlation of coagulation indicators with inflammatory factors was analyzed.</p><p><b>RESULTS</b>The values of TM/TAT/PIC/D-D/FDP in thromboembolism group were statistically significantly higher than those in control group (P<0.05). However, the t-PAIC values were not significantly different (P>0.05), and 3 inflammatory factors (PCT/CRP/IL-6) in thromboembolism patients were significantly higher than those in control group (P<0.05). The correlation analysis suggested that the correlation coefficients of TM with PCT, CRP and IL-6 were 0.288, 0.249 and 0.270, respectively (P<0.05).</p><p><b>CONCLUSION</b>The critical patients with thromboembolism show an obviously higher systemic inflammatory response, and accompany with coagulation dysfunction. There is a network relationship between inflammation and coagulation, the interaction of inflammatory factors with coagulation indicators promotes thromboembolism and inflammation.</p>

Research progress in culture and in vivo and in vitro HBV infection of primary hepatocytes of tree shrews / 临床肝胆病杂志

HBV is a major health problem faced by human beings. The study of the mechanism of HBV infection has been a key point in this field. To carry out the related research on HBV, establishment of simple and available in vivo and in vitro infection models is the trend. Due to the close relationship between tree shrews and human/primates, the tree shrew model of HBV infection has attracted more and more attention. This review summarizes recent research, both at home and abroad, about in vivo and in vitro HBV infection in tree shrews, especially the research progress in in vitro culture of primary hepatocytes of tree shrews, and looks to the future research directions as well.

Research progress in culture and in vivo and in vitro HBV infection of primary hepatocytes of tree shrews / 临床肝胆病杂志

HBV is a major health problem faced by human beings. The study of the mechanism of HBV infection has been a key point in this field. To carry out the related research on HBV, establishment of simple and available in vivo and in vitro infection models is the trend. Due to the close relationship between tree shrews and human/primates, the tree shrew model of HBV infection has attracted more and more attention. This review summarizes recent research, both at home and abroad, about in vivo and in vitro HBV infection in tree shrews, especially the research progress in in vitro culture of primary hepatocytes of tree shrews, and looks to the future research directions as well.

New advances in tree shrew model in experimental studies of hepatitis B virus / 临床肝胆病杂志

Hepatitis B virus (HBV) infection is the main cause of liver fibrosis, cirrhosis, and hepatocellular carcinoma, and it is also a major health problem around the world. How to establish an efficient, reliable, and standardized animal model of chronic HBV infection is essential to the study of the pathogenesis and prevention strategies for HBV infection. This review summarizes the general research and new advances in using tree shrews as the model of HBV infection. We believe that tree shrews, as lower primates, will provide a vital platform and have a huge potential for building a proper animal model in the future, and could become the essential animal model for simulating the process of HBV infection in humans.

Improved anti-tumor efficiency against prostate cancer by docetaxel-loaded PEG-PCL micelles / 华中科技大学学报（医学）（英德文版）

This study primarily focused on the systematic assessment of both in vitro and in vivo anti-tumor effects of docetaxel-loaded polyethylene glycol (PEG)2000-polycaprolactone (PCL)2600 micelles on hormone-refractory prostate cancer (HRPC). By using solvent evaporation method, PEG-PCL was chosen to prepare doxetaxel (DTX)-loaded mPEG-PCL micelles (DTX-PMs), with the purpose of eliminating side effects of the commercial formulation (Tween 80) and prolonging the blood circulation time. The prepared DTX-PMs had an average particle size of 25.19±2.36 nm, a zeta potential of 0.64±0.15 mV, a polydispersity index of 0.56±0.03, a drug loading of (8.72±1.05)%, and an encapsulation efficiency of (98.1±8.4)%. In vitro cytotoxicity studies indicated that DTX-PMs could effectively kill LNCap-C4-2B cells and show a dose- and time-dependent efficacy. The hemolysis test showed that DTX-PMs had less hemocytolysis than the commercial product of Duopafei®. A sustained in vitro release behavior and prolonged circulation time in blood vessels were observed in the DTX-PMs. Furthermore, when compared with Duopafei®, the DTX-PMs dramatically reduced the prostate specific antigen (PSA) level and tumor growth of prostate tumor-bearing nude mice in vivo. In conclusion, the DTX-PMs can lower systemic side effects, improve anti-tumor activity with prolonged blood circulation time, and will bring an alternative to patients with HRPC.

Improved anti-tumor efficiency against prostate cancer by docetaxel-loaded PEG-PCL micelles / 华中科技大学学报（医学）（英德文版）

This study primarily focused on the systematic assessment of both in vitro and in vivo anti-tumor effects of docetaxel-loaded polyethylene glycol (PEG)2000-polycaprolactone (PCL)2600 micelles on hormone-refractory prostate cancer (HRPC). By using solvent evaporation method, PEG-PCL was chosen to prepare doxetaxel (DTX)-loaded mPEG-PCL micelles (DTX-PMs), with the purpose of eliminating side effects of the commercial formulation (Tween 80) and prolonging the blood circulation time. The prepared DTX-PMs had an average particle size of 25.19±2.36 nm, a zeta potential of 0.64±0.15 mV, a polydispersity index of 0.56±0.03, a drug loading of (8.72±1.05)%, and an encapsulation efficiency of (98.1±8.4)%. In vitro cytotoxicity studies indicated that DTX-PMs could effectively kill LNCap-C4-2B cells and show a dose- and time-dependent efficacy. The hemolysis test showed that DTX-PMs had less hemocytolysis than the commercial product of Duopafei®. A sustained in vitro release behavior and prolonged circulation time in blood vessels were observed in the DTX-PMs. Furthermore, when compared with Duopafei®, the DTX-PMs dramatically reduced the prostate specific antigen (PSA) level and tumor growth of prostate tumor-bearing nude mice in vivo. In conclusion, the DTX-PMs can lower systemic side effects, improve anti-tumor activity with prolonged blood circulation time, and will bring an alternative to patients with HRPC.

Urethroplasty with pedunculated preputial flap and testicular tunica vaginalis for long anterior urethral stricture: A case report and review of the literature / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the treatment of urethral stricture.</p><p><b>METHODS</b>We retrospectively studied the clinical data of 1 case of long anterior urethral stricture treated by urethroplasty with pedunculated preputial flap and testicular tunica vaginalis, and summarized the treatment of the disease with review of the relevant literature.</p><p><b>RESULTS</b>The operation was smooth and successful, and no such complications as fistula and urethral stricture were found during the follow-up.</p><p><b>CONCLUSION</b>Urethroplasty with pedunculated preputial flap and testicular tunica vaginalis as a substitute is feasible for the treatment of urethral stricture. The key to a successful operation is the proper choice of a urethral substitute.</p>

Psychological Factors Are Closely Associated with the Bell's Palsy: A Case-control Study / 华中科技大学学报（医学）（英德文版）

To observe the differences in psychological status between Bell's palsy (BP) patients and healthy subjects,and to examine the relationship between psychological factors and the severity of BP,we conducted a case-control,multi-center clinical investigation.A total of 695 subjects were assigned to the case group (n=355) and the control group (n=340).House-Brackmann grading system and Facial Disability Index (FDI) were adopted to assess the BP patients; Kessler Psychological Distress Scale (K10) and 16 Personality Factor (16PF) scale were employed to evaluate the psychological distress and personality profiles of all subjects.Two independent samples t test was used to compare the differences between cases and controls,and to compare the differences among different BP patients.Pearson correlation analysis was used to examine the relationship between psychological factors and severity of facial paralysis.The results showed that psychological distress (K1 0) in case group (27.09±5.80) was significantly higher than that in control group (13.43±3.02) (t-37.219,P0.000).The scores of personality factor Warmth (A),Openness to Change (Q1),Self-Reliance (Q2) were lower in cases than in the controls (P＜0.01,P＜0.05,P＜0.05,respectively),whereas the scores of Sensitivity (I),Vigilance (L),Apprehension (O),and Tension (Q4) were significantly higher in cases than in the controls (P＜0.05,P＜0.01,P＜0.01,P＜0.01,respectively).In addition,the psychological distress was significantly higher in female patients,severe (HB score Ⅳ-Ⅵ) patients,and subacute (onset time 72-168 h) patients compared with that in male patients,mild (HB score Ⅰ -Ⅲ) patients,and acute (onset time≤72 h) patients (P＜0.05).The scores of personality factor in female patients,severe patients,and subacute patients were also significantly different from male patients,mild patients,and acute patients (P＜0.05).The result of Pearson correlation analysis showed that psychological factors (K10,personality A,F,L,N,O,Q4) were closely related to HB scores.We are led to conclude that the psychological status between BP patients and healthy people are different; psychological distress and personality factors are closely associated with severity of facial paralysis.

Study on hydrolysable tannin constituents of seed of Juglans regia II / 中国中药杂志

<p><b>OBJECTIVE</b>To study hydrolysable tannin constituents of the seed of Juglans regia.</p><p><b>METHOD</b>The chemical constituents were isolated by Diaion HP-20, Toyopaerl HW-40 and MCI gel CHP-20P column chromatogramphy and identified by physicochemical identification and spectral data.</p><p><b>RESULT</b>Six compounds obtained from the 70% ethanol extract were identified as 1, 2, 3, 4, 6-penta-O-galloyl-3-D-glucose (1), rugosin C (2), 1, 2, 3, 6-tetra-O-galloyl-3-D-glugose (3), tellimagrandin II (4), casuarictin (5), 1-degalloylrugosin F (6).</p><p><b>CONCLUSION</b>All compouds were isolated from the seeds of J. regia for the first time.</p>

Surgical Treatment of Thoracolumbar Spine Burst Fracture by Anterior Decompression Bone Grafting,Internal Fixation and Imabsolute Paralysis / 昆明医科大学学报

Objective To sum up the surgical characteristics,indications and curative effects of burst fracture by anterior decompression,bone grafting and internal fixation.Methods Since Sep 2004,6 cases with the average age of 32 who were with thoracolumbar spine burst fracture and spinal cord injury of 12-day mean time,were treated with anterior decompression,bone grafting and Z-plate steel plate internal fixation.Before the operation,pateients were classified according to the ASIA classification: 4 cases of grade C,2 cases of grade D;Kyphotic angle(Cobb)15~40?;and vertebral canal 50%~60%.Results The follow-up lasted from 6 to 16 months(average 11 months) and it was found that incision recovered better,no infection of thoracic,abdomen,no grafting loosing or braking occurred.According to the ASIA classification,the functional recovery of spinal cord was 1~2 grade.The Denis assessment showed 5 cases was light back pain,1 case needed medication from time to time but didn't affect the life and work and spine was in order.Conclusion Surgical treatment of thoracolumbar fracture by anterior decompression,bone grafting,internal fixation can provide sufficient decompression,high rate of fusion of the implanted bone,stability in the spine and vertebral body,better recovery of neurological function and satisfied curative effects.It is the best choice for thoracolumbar burst facture and spinal cord injury.

Studies on hydrolysable tannin constituents in seed of Juglans regia(I) / 中国中药杂志

<p><b>OBJECTIVE</b>To study hydrolysable tannin constituents of the seeds of Juglans regia.</p><p><b>METHOD</b>The chemical constituents were isolated by Diaion HP-20 and Toyopaerl HW-40 MCI gel CHP-20P column chromatogramphy and identified by physicochemical identification and spectral data.</p><p><b>RESULT</b>The compounds obtained from the 70% acetone extract were identified as gemin D (1), casuariin (2), pedunculagin (3), tellimagrandin I (4), rugosin F (5), heterophylliin D (6).</p><p><b>CONCLUSION</b>All other compounds which were isolated from the seeds of J. regia for the first time.</p>

Changes of heart rate variability and impairment of learning and memory induced by cerebral ischemia/reperfusion in rats / 生理学报

The present study was designed to observe the influence of cerebral ischemia/reperfusion injury on learning and memory in hyperlipidemic rats and estimate the changes of activity of autonomic nervous system. Twenty-three male Wistar rats were randomly divided into three groups, named control group (C group, n=10), hyperlipidemia group (H group, n=6) and hyperlipidemia-ischemia group (HI group, n=7), respectively. The rats in H and HI group were fed a high-fat diet for 2 weeks and the rats in all groups were examined through Morris water maze (MWM) task. The rats in HI group underwent ischemia/reperfusion by 2-vessel occlusion (2-VO) method, and had electrocardiogram (ECG) recording simultaneously. The MWM task and ECG recording were taken again after 7 d of recuperation. The following results were obtained: (1) In the second place navigation performance and probe trial performance, the frequency of memory in quadrant of hidden-platform and memory score decreased significantly in HI group compared to that in C and H groups. (2) The heart rate in HI group decreased slowly after ischemia; the power at high frequency band (HF) reduced gradually, meanwhile the power at middle frequency band (MF) and the ratio of power at MF and HF decreased clearly compared to baseline value. (3) After 7 d of ischemia/reperfusion, the heart rate in HI group was significantly higher than that in H group (P<0.05). While there was no statistical change in the power at MF, the power at HF decreased and the ratio of MF/HF increased significantly (P<0.05). The data demonstrated that ischemia/reperfusion decreased the activity of autonomic nervous system, and the reduction of sympathetic nerve activity was much more than that of vagus nerve activity. The results suggest that the hippocampus neuron injury caused by ischemia induces cognitive disorder and imbalance of vago-sympathetic nerve activity accompanied by vagus nerve suppression.

Expression of cyclooxygenase-2 and vascular endothelial growth factor in human prostate cancer and its significance / 中华男科学杂志

<p><b>OBJECTIVE</b>To evaluate expressions of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in primary prostate cancer and its clinical significance.</p><p><b>METHODS</b>Expressions of COX-2 and VEGF were detected by immunohistochemical assay in tissues of 40 prostate cancer and 10 benign prostatic hyperplasia samples.</p><p><b>RESULTS</b>COX-2 and VEGF levels in prostate cancer were much higher than those in BPH. The degrees of cancer malignancy and invasion positively correlated with the expressions of COX-2 and VEGF. COX-2 level positively correlated with VEGF level.</p><p><b>CONCLUSION</b>The abnormal expression of COX-2 plays an important role in the development of primary prostate cancer. COX-2 and VEGF are good molecular markers of prostate cancer which are hopeful to be used for the assistant diagnosis and the prediction of prognosis of prostate cancer.</p>

Morphologic characteristics and identification methods of seeds in Astragalus membranaceus var.mongholicus and A.membranaceus / 中草药

Objective To provide a method for identifying the seeds of Astragalus membranaceus var.mongholicus(AMM) and A.membranaceus(AM) and to provide basic research data for establishing relevant Standard Operating Practice(SOP) in accordance with GAP of traditional Chinese medicinal materials.Methods The seed morphologic characteristics and microcosmic structures were observed by eyes,light microscopes,and electron microscopes;the seed germination rates of AMM and AM were also compared.Results There was no obvious discrimination of the seed morphology between AMM and AM.There was obvious discrimination on the characteristic of germination-hole,the microcosmic structures of seed-umbilici and seed-coats of seeds between AMM and AM.The hard seed percentage for AMM was higher than that of AM,and its sprouting was not even and sprouting peak appeared later than that of AM.(Conclusion) The seeds of AMM and AM can be identified accuratly with electron microscopes.The patterns of germination-hole,microcosmic structures of seed-umbilici and seed-coats can be used as indices to identify the seeds of AMM and AM.The hard seed percentage and germination characteristics can be used to(identify) the seeds of AMM and AM subsidiarily.